Impact of miRNA modulation on cerebral cells in an in vitro model of hypoxia reoxygenation

miRNAs have been implicated as key modulators of pathogenesis in numerous diseases. This project aims to assess the role of a specific miRNA, selected from a previously performed openarray, following hypoxic injury in cerebral cells. The study will utilise a neuronal and brain-derived endothelial cell line in an optimised model of in vitro hypoxia / reoxygenation. Using pre-miR’s to increase and antagomiRs to knockdown the endogenous levels of the specific miRNA, the mechanistic action of the miRNA can be assessed using a number of previously optimised assays of oxidative stress, apoptosis and cell proliferation.